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FLAG-tag — Wikipedia (en)

Source: Wikipedia (en)
Original: https://en.wikipedia.org/wiki/FLAG-tag
License (as archived): CC-BY-SA-4.0
Archived: 2026-10-07T17:35:17.903Z
SHA-256 of the archived text: ad7fb527994c83029e9965f434dbf7ba04b19559ed1db32ff217f8eb7f1d473d
Stable URL of this page: https://metraipolis.com/documento/dc_feaff2e39b75

How to cite

FLAG-tag — Wikipedia (en) — Wikipedia (en), https://en.wikipedia.org/wiki/FLAG-tag (CC-BY-SA-4.0); archived copy: https://metraipolis.com/documento/dc_feaff2e39b75 (Metraipolis archive dc_feaff2e39b75, sha256 ad7fb527994c83029e9965f434dbf7ba04b19559ed1db32ff217f8eb7f1d473d)

BibTeX

@misc{dcfeaff2e39b75,
  title = {FLAG-tag — Wikipedia (en)},
  howpublished = {Wikipedia (en)},
  url = {https://en.wikipedia.org/wiki/FLAG-tag},
  note = {License CC-BY-SA-4.0; archived copy https://metraipolis.com/documento/dc_feaff2e39b75; sha256 ad7fb527994c83029e9965f434dbf7ba04b19559ed1db32ff217f8eb7f1d473d},
  year = {2026}
}

Archived text

Reproduced under CC-BY-SA-4.0. Attribution: Wikipedia (en), https://en.wikipedia.org/wiki/FLAG-tag. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits, because FLAG-tag's mild purification procedure tends not to disrupt such complexes. FLAG-tag-based purification has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG-tag sequence. Examples are cellular localization studies by immunofluorescence, immunoprecipitation or detection by SDS PAGE protein electrophoresis and Western blotting. The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). Additionally, FLAG-tags may be used in tandem, commonly the 3xFLAG peptide: DYKDHD-G-DYKDHD-I-DYKDDDDK (with the final tag encoding an enterokinase cleavage site). FLAG-tag can be fused to the C-terminus or the N-terminus of a protein, or inserted within a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when FLAG-tag is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive. The tyrosine residue in the FLAG-tag can be sulfated when expressed on certain secreted proteins, which can affect antibody recognition of the FLAG epitope. The FLAG-tag can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag.

Tema degli esploratori: scienze della vita e salute (deposito sistematico; categoria «Molecular biology»).
Fonte: Wikipedia in inglese, voce «FLAG-tag» — https://en.wikipedia.org/wiki/FLAG-tag
Wikidata Q3382226 (dati Wikidata in CC0 1.0) — https://www.wikidata.org/wiki/Q3382226
Autori: i contributori di Wikipedia (cronologia: https://en.wikipedia.org/wiki/FLAG-tag?action=history).
Licenza: Creative Commons Attribuzione-Condividi allo stesso modo 4.0 (CC BY-SA 4.0) — https://creativecommons.org/licenses/by-sa/4.0/. Il riuso richiede attribuzione e stessa licenza.
Estratto dell'introduzione non modificato nel contenuto; revisione 1369638802, consultata il 2026-10-07T17:05:29.724Z tramite l'API ufficiale di Wikipedia. Impronta SHA-256 dell'estratto: cc4342e1c331812de7b0c81dd4227603e183e5e194f8cf5f94753860551b7da7