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Electrophoretic mobility shift assay — Wikipedia (en)

Source: Wikipedia (en)
Original: https://en.wikipedia.org/wiki/Electrophoretic_mobility_shift_assay
License (as archived): CC-BY-SA-4.0
Archived: 2026-10-07T16:11:47.550Z
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Electrophoretic mobility shift assay — Wikipedia (en) — Wikipedia (en), https://en.wikipedia.org/wiki/Electrophoretic_mobility_shift_assay (CC-BY-SA-4.0); archived copy: https://metraipolis.com/documento/dc_ef32efd6e2fb (Metraipolis archive dc_ef32efd6e2fb, sha256 5d00221ae4dbd7e1a9fc614cc9eadcb6689d33a21e33401c96718c4c12d4bf00)

BibTeX

@misc{dcef32efd6e2fb,
  title = {Electrophoretic mobility shift assay — Wikipedia (en)},
  howpublished = {Wikipedia (en)},
  url = {https://en.wikipedia.org/wiki/Electrophoretic_mobility_shift_assay},
  note = {License CC-BY-SA-4.0; archived copy https://metraipolis.com/documento/dc_ef32efd6e2fb; sha256 5d00221ae4dbd7e1a9fc614cc9eadcb6689d33a21e33401c96718c4c12d4bf00},
  year = {2026}
}

Archived text

Reproduced under CC-BY-SA-4.0. Attribution: Wikipedia (en), https://en.wikipedia.org/wiki/Electrophoretic_mobility_shift_assay. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

An electrophoretic mobility shift assay (EMSA) or mobility shift electrophoresis, also referred as a gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a common affinity electrophoresis technique used to study protein–DNA or protein–RNA interactions. This procedure can determine if a protein or mixture of proteins is capable of binding to a given DNA or RNA sequence, and can sometimes indicate if more than one protein molecule is involved in the binding complex. Gel shift assays are often performed in vitro concurrently with DNase footprinting, primer extension, and promoter-probe experiments when studying transcription initiation, DNA gang replication, DNA repair or RNA processing and maturation, as well as pre-mRNA splicing. Although precursors can be found in earlier literature, most current assays are based on methods described by Garner and Revzin and Fried and Crothers.

Tema degli esploratori: scienze della vita e salute (deposito sistematico; categoria «Molecular biology»).
Fonte: Wikipedia in inglese, voce «Electrophoretic mobility shift assay» — https://en.wikipedia.org/wiki/Electrophoretic_mobility_shift_assay
Wikidata Q903596 (dati Wikidata in CC0 1.0) — https://www.wikidata.org/wiki/Q903596
Autori: i contributori di Wikipedia (cronologia: https://en.wikipedia.org/wiki/Electrophoretic_mobility_shift_assay?action=history).
Licenza: Creative Commons Attribuzione-Condividi allo stesso modo 4.0 (CC BY-SA 4.0) — https://creativecommons.org/licenses/by-sa/4.0/. Il riuso richiede attribuzione e stessa licenza.
Estratto dell'introduzione non modificato nel contenuto; revisione 1296790607, consultata il 2026-10-07T15:44:50.433Z tramite l'API ufficiale di Wikipedia. Impronta SHA-256 dell'estratto: c56b5505c727a15d8006e24e432741c55251d0d3a355b9e49c4230f2777c7a92