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Characterization of a Neisseria gonorrhoeae Ciprofloxacin panel for an antimicrobial resistant Isolate Bank — Europe PMC PMID:35271599

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/35271599
DOI: 10.1371/journal.pone.0264149
License (as archived): CC0-1.0
Archived: 2026-10-31T08:03:18.220Z
SHA-256 of the archived text: 2f8908eff5ef94576f7c642ad3455fb13a6f18e7888959c4c2368860176553a4
Stable URL of this page: https://metraipolis.com/documento/dc_e607b25bb501

How to cite

Characterization of a Neisseria gonorrhoeae Ciprofloxacin panel for an antimicrobial resistant Isolate Bank — Europe PMC PMID:35271599 — Europe PMC (en), https://europepmc.org/article/MED/35271599 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_e607b25bb501 (Metraipolis archive dc_e607b25bb501, sha256 2f8908eff5ef94576f7c642ad3455fb13a6f18e7888959c4c2368860176553a4)

BibTeX

@misc{dce607b25bb501,
  title = {Characterization of a Neisseria gonorrhoeae Ciprofloxacin panel for an antimicrobial resistant Isolate Bank — Europe PMC PMID:35271599},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/35271599},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_e607b25bb501; sha256 2f8908eff5ef94576f7c642ad3455fb13a6f18e7888959c4c2368860176553a4},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/35271599. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Objectives: Neisseria gonorrhoeae (gonococcus) infection is one of the most commonly reported nationally notifiable conditions in the United States. Gonococcus has developed antimicrobial resistance to each previously used antibiotic for gonorrhea therapy. However, some isolates may be still susceptible to no longer recommended, yet still effective antibiotics. This in turn suggests that targeted therapy could slow resistance development to currently recommended empirical treatments. We curated a gonococcal Ciprofloxacin Antibiotic Resistance Isolate Bank panel (Cipro-panel) as a tool for validating or developing new tests to determine ciprofloxacin susceptibility. Method: The Cipro-panel was selected using whole genome sequencing, bioinformatic tools, and antimicrobial susceptibility testing (AST) data. Isolates were further selected based on nucleotide variations in gyrA and parC genes. Results: We selected 14 unique N. gonorrhoeae isolates from the 2006-2012 Gonococcal Isolate Surveillance Project (GISP) collection. They represented a wide range of antimicrobial susceptibility to ciprofloxacin and commonly observed nucleotide variations of gyrA and parC genes. This Cipro-panel consists of 5 isolates with resistant phenotypes (MIC > = 1 μg/mL), 8 isolates with susceptible phenotypes (MIC < = 0.06 μg/mL), and 1 isolate falling in the Clinical and Laboratory Standards Institute defined intermediate range. Among the gyrA variations we observed a total of 18 SNPs. Four positions had nonsynonymous changes (nucleotide positions 272, 284, 1093, and 1783). The first two positions (272 and 284) have been linked previously with resistance to ciprofloxacin (i.e. amino acid positions 91 and 95). For the parC gene, we observed a total of 21 possible SNPs. Eight of those SNPs resulted in non-synonymous amino acid changes. One location (amino acid 87) has been previously reported to be associated with ciprofloxacin resistance. Conclusions: This Cipro-Panel is useful for researchers interested in developing clinical tests related to ciprofloxacin. It could also provide additional choices for validation, quality assurance purposes and improve antibiotic usage.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PloS one, 2022 (prima pubblicazione 2022-03-10).
Identificativi: PMID 35271599 · PMCID PMC8912267 · DOI 10.1371/journal.pone.0264149.
Descrittori MeSH principali: Neisseria gonorrhoeae; Gonorrhea.
Pagina: https://europepmc.org/article/MED/35271599 — https://doi.org/10.1371/journal.pone.0264149. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
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