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Biochemical and immunological characterization of an ETEC CFA/I adhesin cholera toxin B subunit chimera — Europe PMC PMID:32176708

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/32176708
DOI: 10.1371/journal.pone.0230138
License (as archived): CC0-1.0
Archived: 2026-11-09T05:27:44.998Z
SHA-256 of the archived text: 65bb9ebed477bf4417cd2e050c419a8e5a5bb9ed05e31a11ba9e6e0e55ba530d
Stable URL of this page: https://metraipolis.com/documento/dc_e2db07fdcd69

How to cite

Biochemical and immunological characterization of an ETEC CFA/I adhesin cholera toxin B subunit chimera — Europe PMC PMID:32176708 — Europe PMC (en), https://europepmc.org/article/MED/32176708 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_e2db07fdcd69 (Metraipolis archive dc_e2db07fdcd69, sha256 65bb9ebed477bf4417cd2e050c419a8e5a5bb9ed05e31a11ba9e6e0e55ba530d)

BibTeX

@misc{dce2db07fdcd69,
  title = {Biochemical and immunological characterization of an ETEC CFA/I adhesin cholera toxin B subunit chimera — Europe PMC PMID:32176708},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/32176708},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_e2db07fdcd69; sha256 65bb9ebed477bf4417cd2e050c419a8e5a5bb9ed05e31a11ba9e6e0e55ba530d},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/32176708. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Surface-expressed colonization factors and their subunits are promising candidates for inclusion into a multivalent vaccine targeting enterotoxigenic Escherichia coli (ETEC), a leading cause of acute bacterial diarrhea in developing regions. However, soluble antigens are often poorly immunogenic in the absence of an adjuvant. We show here that the serum immune response to CfaE, the adhesin of the ETEC colonization factor CFA/I, can be enhanced in BALB/c mice by immunization with a chimeric antigen containing CfaE and pentameric cholera toxin B subunit (CTB) of cholera toxin from Vibrio cholerae. We constructed this antigen by replacing the coding sequence for the A1 domain of the cholera toxin A subunit (CTA) with the sequence of donor strand complemented CfaE (dscCfaE) within the cholera toxin operon, resulting in a dscCfaE-CTA2 fusion. After expression, via non-covalent interactions between CTA2 and CTB, the fusion and CTB polypeptides assemble into a complex containing a single dscCfaE-CTA2 protein bound to pentameric CTB (dscCfaE-CTA2/CTB). This holotoxin-like chimera retained the GM1 ganglioside binding activity of CTB, as well as the ability of CfaE to mediate the agglutination of bovine red blood cells when adsorbed to polystyrene beads. When administered intranasally to mice, the presence of CTB in the chimera significantly increased the serum immune response to CfaE compared to dscCfaE alone, stimulating a response similar to that obtained with a matched admixture of dscCfaE and CTB. However, by the orogastric route, immunization with the chimera elicited a superior functional immune response compared to an equivalent admixture of dscCfaE and CTB, supporting further investigation of the chimera as an ETEC vaccine candidate.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PloS one, 2020 (prima pubblicazione 2020-03-16).
Identificativi: PMID 32176708 · PMCID PMC7075575 · DOI 10.1371/journal.pone.0230138.
Descrittori MeSH principali: Cholera Toxin; Fimbriae Proteins; Recombinant Fusion Proteins; Escherichia coli Vaccines; Enterotoxigenic Escherichia coli.
Pagina: https://europepmc.org/article/MED/32176708 — https://doi.org/10.1371/journal.pone.0230138. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-11-09T05:24:26.210Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.