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Genetic typing of isolates of Rickettsia typhi — Europe PMC PMID:35639778

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/35639778
DOI: 10.1371/journal.pntd.0010354
License (as archived): CC0-1.0
Archived: 2026-10-18T09:52:43.801Z
SHA-256 of the archived text: d644c151415af6bc1f1e66ae175aff6bd711bfc00e9b9ed4dd9f72ee9d10556a
Stable URL of this page: https://metraipolis.com/documento/dc_db46ce72d3bb

How to cite

Genetic typing of isolates of Rickettsia typhi — Europe PMC PMID:35639778 — Europe PMC (en), https://europepmc.org/article/MED/35639778 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_db46ce72d3bb (Metraipolis archive dc_db46ce72d3bb, sha256 d644c151415af6bc1f1e66ae175aff6bd711bfc00e9b9ed4dd9f72ee9d10556a)

BibTeX

@misc{dcdb46ce72d3bb,
  title = {Genetic typing of isolates of Rickettsia typhi — Europe PMC PMID:35639778},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/35639778},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_db46ce72d3bb; sha256 d644c151415af6bc1f1e66ae175aff6bd711bfc00e9b9ed4dd9f72ee9d10556a},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/35639778. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Murine typhus, which is caused by Rickettsia typhi, has a wide range of clinical manifestations. It has a low mortality rate but may result in meningoencephalitis and interstitial pneumonia in severe cases. Comparisons of complete genome sequences of R. typhi isolates from North Carolina, USA (Wilmington), Myanmar (B9991PP), and Thailand (TH1527) identified only 26 single nucleotide polymorphism (SNP) and 7 insertion-deletion (INDEL) sites in these highly syntenic genomes. Assays were developed to further define the distribution of these variant sites among 15 additional isolates of R. typhi with different histories from Asia, the USA, and Africa. Mismatch amplification mutation assays (MAMA) were validated for 22 SNP sites, while the 7 INDEL sites were analyzed directly on agarose gels. Six SNP types, 9 INDEL types, 11 total types were identified among these 18 isolates. Replicate DNA samples as well as comparisons of isolates with different passage and source histories gave consistent genetic typing profiles. Comparison of the SNP and INDEL markers to R. typhi's nearest neighbor Rickettsia prowazekii demonstrated that the majority of the SNPs represent intra-species variation that arose post divergence of these two species while several INDEL sites also exhibited intraspecies variability among the R. prowazekii genomes that have been completely sequenced. The assays for the presence of these SNP and INDEL sites, particularly the latter, comprise a low technology gel method for consistently distinguishing R. typhi and R. prowazekii as well as for differentiating genetic types of R. typhi.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PLoS neglected tropical diseases, 2022 (prima pubblicazione 2022-05-31).
Identificativi: PMID 35639778 · PMCID PMC9203007 · DOI 10.1371/journal.pntd.0010354.
Descrittori MeSH principali: Rickettsia; Rickettsia prowazekii; Typhus, Endemic Flea-Borne.
Pagina: https://europepmc.org/article/MED/35639778 — https://doi.org/10.1371/journal.pntd.0010354. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-10-18T09:47:44.169Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.