Metraipolis › archive › dc_db019e575342

Tissue specific diversification, virulence and immune response to Mycobacterium bovis BCG in a patient with an IFN-γ R1 deficiency — Europe PMC PMID:33356838

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/33356838
DOI: 10.1080/21505594.2020.1848108
License (as archived): CC0-1.0
Archived: 2026-11-06T18:09:31.134Z
SHA-256 of the archived text: 1dde1680eed356c6e4357890f329def841d0a6afff0b86081bff969b36cd4432
Stable URL of this page: https://metraipolis.com/documento/dc_db019e575342

How to cite

Tissue specific diversification, virulence and immune response to Mycobacterium bovis BCG in a patient with an IFN-γ R1 deficiency — Europe PMC PMID:33356838 — Europe PMC (en), https://europepmc.org/article/MED/33356838 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_db019e575342 (Metraipolis archive dc_db019e575342, sha256 1dde1680eed356c6e4357890f329def841d0a6afff0b86081bff969b36cd4432)

BibTeX

@misc{dcdb019e575342,
  title = {Tissue specific diversification, virulence and immune response to Mycobacterium bovis BCG in a patient with an IFN-γ R1 deficiency — Europe PMC PMID:33356838},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/33356838},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_db019e575342; sha256 1dde1680eed356c6e4357890f329def841d0a6afff0b86081bff969b36cd4432},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/33356838. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Summary : We characterized Mycobacterium bovis BCG isolates found in lung and brain samples from a previously vaccinated patient with IFNγR1 deficiency. The isolates collected displayed distinct genomic and phenotypic features consistent with host adaptation and associated changes in antibiotic susceptibility and virulence traits. Background : We report a case of a patient with partial recessive IFNγR1 deficiency who developed disseminated BCG infection after neonatal vaccination (BCG-vaccine). Distinct M. bovis BCG-vaccine derived clinical strains were recovered from the patient's lungs and brain. Methods : BCG strains were phenotypically (growth, antibiotic susceptibility, lipid) and genetically (whole genome sequencing) characterized. Mycobacteria cell infection models were used to assess apoptosis, necrosis, cytokine release, autophagy, and JAK-STAT signaling. Results : Clinical isolates BCG-brain and BCG-lung showed distinct Rv0667 rpoB mutations conferring high- and low-level rifampin resistance; the latter displayed clofazimine resistance through Rv0678 gene (MarR-like transcriptional regulator) mutations. BCG-brain and BCG-lung showed mutations in fadA2, fadE5, and mymA operon genes, respectively. Lipid profiles revealed reduced levels of PDIM in BCG-brain and BCG-lung and increased TAGs and Mycolic acid components in BCG-lung, compared to parent BCG-vaccine. In vitro infected cells showed that the BCG-lung induced a higher cytokine release, necrosis, and cell-associated bacterial load effect when compared to BCG-brain; conversely, both strains inhibited apoptosis and altered JAK-STAT signaling. Conclusions : During a chronic-disseminated BCG infection, BCG strains can evolve independently at different sites likely due to particular microenvironment features leading to differential antibiotic resistance, virulence traits resulting in dissimilar responses in different host tissues.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: Virulence, 2020 (prima pubblicazione 2020-12-01).
Identificativi: PMID 33356838 · PMCID PMC7781554 · DOI 10.1080/21505594.2020.1848108.
Parole chiave degli autori: Tuberculosis; BCG; Antibiotic Resistance; Immunodeficiency; Interferon-γ; Mendelian Susceptibility To Mycobacterial Diseases.
Pagina: https://europepmc.org/article/MED/33356838 — https://doi.org/10.1080/21505594.2020.1848108. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-11-06T18:06:08.485Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.