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The alphaherpesvirus conserved pUS10 is important for natural infection and its expression is regulated by the conserved Herpesviridae protein kinase (CHPK) — Europe PMC PMID:36749787

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/36749787
DOI: 10.1371/journal.ppat.1010959
License (as archived): CC0-1.0
Archived: 2026-10-07T18:44:51.579Z
SHA-256 of the archived text: cb6a74a447fa7e4ac5f9a3d503338624cf4420f19d0f6fb60dcd6fe6d7a396fb
Stable URL of this page: https://metraipolis.com/documento/dc_d4e930342e64

How to cite

The alphaherpesvirus conserved pUS10 is important for natural infection and its expression is regulated by the conserved Herpesviridae protein kinase (CHPK) — Europe PMC PMID:36749787 — Europe PMC (en), https://europepmc.org/article/MED/36749787 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_d4e930342e64 (Metraipolis archive dc_d4e930342e64, sha256 cb6a74a447fa7e4ac5f9a3d503338624cf4420f19d0f6fb60dcd6fe6d7a396fb)

BibTeX

@misc{dcd4e930342e64,
  title = {The alphaherpesvirus conserved pUS10 is important for natural infection and its expression is regulated by the conserved Herpesviridae protein kinase (CHPK) — Europe PMC PMID:36749787},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/36749787},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_d4e930342e64; sha256 cb6a74a447fa7e4ac5f9a3d503338624cf4420f19d0f6fb60dcd6fe6d7a396fb},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/36749787. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Conserved Herpesviridae protein kinases (CHPK) are conserved among all members of the Herpesviridae. Herpesviruses lacking CHPK propagate in cell culture at varying degrees, depending on the virus and cell culture system. CHPK is dispensable for Marek's disease herpesvirus (MDV) replication in cell culture and experimental infection in chickens; however, CHPK-particularly its kinase activity-is essential for horizontal transmission in chickens, also known as natural infection. To address the importance of CHPK during natural infection in chickens, we used liquid chromatography-tandem mass spectrometry (LC-MS/MS) based proteomics of samples collected from live chickens. Comparing modification of viral proteins in feather follicle epithelial (FFE) cells infected with wildtype or a CHPK-null virus, we identified the US10 protein (pUS10) as a potential target for CHPK in vivo. When expression of pUS10 was evaluated in cell culture and in FFE skin cells during in vivo infection, pUS10 was severely reduced or abrogated in cells infected with CHPK mutant or CHPK-null viruses, respectively, indicating a potential role for pUS10 in transmission. To test this hypothesis, US10 was deleted from the MDV genome, and the reconstituted virus was tested for replication, horizontal transmission, and disease induction. Our results showed that removal of US10 had no effect on the ability of MDV to transmit in experimentally infected chickens, but disease induction in naturally infected chickens was significantly reduced. These results show CHPK is necessary for pUS10 expression both in cell culture and in the host, and pUS10 is important for disease induction during natural infection.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PLoS pathogens, 2023 (prima pubblicazione 2023-02-07).
Identificativi: PMID 36749787 · PMCID PMC9946255 · DOI 10.1371/journal.ppat.1010959.
Descrittori MeSH principali: Herpesviridae; Alphaherpesvirinae; Marek Disease.
Pagina: https://europepmc.org/article/MED/36749787 — https://doi.org/10.1371/journal.ppat.1010959. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-10-07T18:07:41.140Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.