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5-base sequencing — Wikipedia (en)

Source: Wikipedia (en)
Original: https://en.wikipedia.org/wiki/5-base_sequencing
License (as archived): CC-BY-SA-4.0
Archived: 2026-11-06T17:33:16.152Z
SHA-256 of the archived text: 6d0b54c80ae0e6d24a440edba0fbeffbe1d10471a72543ea8448060cbe0c4ece
Stable URL of this page: https://metraipolis.com/documento/dc_cfbb6fb439f0

How to cite

5-base sequencing — Wikipedia (en) — Wikipedia (en), https://en.wikipedia.org/wiki/5-base_sequencing (CC-BY-SA-4.0); archived copy: https://metraipolis.com/documento/dc_cfbb6fb439f0 (Metraipolis archive dc_cfbb6fb439f0, sha256 6d0b54c80ae0e6d24a440edba0fbeffbe1d10471a72543ea8448060cbe0c4ece)

BibTeX

@misc{dccfbb6fb439f0,
  title = {5-base sequencing — Wikipedia (en)},
  howpublished = {Wikipedia (en)},
  url = {https://en.wikipedia.org/wiki/5-base_sequencing},
  note = {License CC-BY-SA-4.0; archived copy https://metraipolis.com/documento/dc_cfbb6fb439f0; sha256 6d0b54c80ae0e6d24a440edba0fbeffbe1d10471a72543ea8448060cbe0c4ece},
  year = {2026}
}

Archived text

Reproduced under CC-BY-SA-4.0. Attribution: Wikipedia (en), https://en.wikipedia.org/wiki/5-base_sequencing. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

5-base sequencing refers to the detection of five nucleotide bases in DNA, including the four standard bases (adenine, guanine, cytosine, and thymine) and a modified base, 5-methylcytosine (5mC), an important epigenetic marker. In a typical DNA sequencing experiment, the epigenetic modification is lost during the PCR amplification step and only the base forms of nucleotides are recognized. In a 5-base sequencing based experiment, the information about methylation of cytosines is preserved through chemical or enzymatic modification allowing inference of methylation status during bioinformatics analysis. More recently, nanopore or single-molecule realtime sequencing have enabled direct detection of methylated cytosines without any conversion of the DNA. In October 2025, Illumina announced a proprietary 5-base sequencing solution using an engineered DNA for 5mC detection on top of standard bases. The approach can further be extended to detect 5hmC modification, known as 6-base genome or sequencing.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (deposito sistematico; retro-catalogo: tutte le voci di Wikipedia in inglese, in ordine alfabetico).
Fonte: Wikipedia in inglese, voce «5-base sequencing» — https://en.wikipedia.org/wiki/5-base_sequencing
Wikidata Q135742267: «DNA sequencing method» (dati Wikidata in CC0 1.0) — https://www.wikidata.org/wiki/Q135742267
Autori: i contributori di Wikipedia (cronologia: https://en.wikipedia.org/wiki/5-base_sequencing?action=history).
Licenza: Creative Commons Attribuzione-Condividi allo stesso modo 4.0 (CC BY-SA 4.0) — https://creativecommons.org/licenses/by-sa/4.0/. Il riuso richiede attribuzione e stessa licenza.
Estratto dell'introduzione non modificato nel contenuto; revisione 1325484583, consultata il 2026-11-06T17:25:39.245Z tramite l'API ufficiale di Wikipedia. Impronta SHA-256 dell'estratto: cc205a698637f067a3124b6a78546a8ef66bbeb8e951ed46953c4023aa8fb492