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Physiologic recovery of Mycobacterium tuberculosis from drug injury: A molecular study of post antibiotic effect in vitro and in vivo — Europe PMC PMID:42018569

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/42018569
DOI: 10.1371/journal.ppat.1014131
License (as archived): CC0-1.0
Archived: 2026-10-06T09:24:35.649Z
SHA-256 of the archived text: 444a07a60a4113dafe84d60e3784f7b366a8612159ec4718e92ab599093d6a2c
Stable URL of this page: https://metraipolis.com/documento/dc_ce549cbef24d

How to cite

Physiologic recovery of Mycobacterium tuberculosis from drug injury: A molecular study of post antibiotic effect in vitro and in vivo — Europe PMC PMID:42018569 — Europe PMC (en), https://europepmc.org/article/MED/42018569 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_ce549cbef24d (Metraipolis archive dc_ce549cbef24d, sha256 444a07a60a4113dafe84d60e3784f7b366a8612159ec4718e92ab599093d6a2c)

BibTeX

@misc{dcce549cbef24d,
  title = {Physiologic recovery of Mycobacterium tuberculosis from drug injury: A molecular study of post antibiotic effect in vitro and in vivo — Europe PMC PMID:42018569},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/42018569},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_ce549cbef24d; sha256 444a07a60a4113dafe84d60e3784f7b366a8612159ec4718e92ab599093d6a2c},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/42018569. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Post-antibiotic effect (PAE) describes the delay in bacterial growth that continues after antibiotics are cleared. The physiologic basis of PAE in Mycobacterium tuberculosis (Mtb) remains poorly understood. Here, we evaluated the long-standing hypothesis that PAE reflects the time required for bacteria to recover from drug-induced physiologic damage by comparing Mtb after varying durations of treatment with the four-drug isoniazid, rifampin, pyrazinamide, ethambutol combination in vitro and in BALB/c mice using two novel molecular readouts of bacterial health. In aerobic axenic culture and in the high-dose aerosol mouse model, quantification of Mtb rRNA synthesis via the RS ratio and Mtb transcriptional profiling via SEARCH-TB revealed that longer drug exposure was associated with greater injury and adaptation during treatment, as well as slower recovery after treatment, i.e., longer PAE. Recovery followed a conserved sequence, from resumption of rRNA synthesis, to broad transcriptional reprogramming, to eventual CFU change. In mice, however, physiologic recovery was markedly slower and less complete than in vitro, indicating longer PAE in the context of immunity. Our observation that PAE in Mtb depends on the duration of drug exposure and correlates with the degree of bacterial injury support the hypothesis that nonlethal physiologic damage contributes to PAE. Our observation that PAE of the standard TB regimen is longer in mice than in vitro indicates that immunity augments PAE for Mtb. Molecular evaluation of bacterial physiology provides a new basis for probing recovery from drug exposure and understanding PAE.

Tema degli esploratori: scienze della vita e salute (ricerca Europe PMC «microbiology», solo open access).
Pubblicazione: PLoS pathogens, 2026 (prima pubblicazione 2026-04-22).
Identificativi: PMID 42018569 · PMCID PMC13128115 · DOI 10.1371/journal.ppat.1014131.
Descrittori MeSH principali: Mycobacterium tuberculosis; Tuberculosis; Antitubercular Agents.
Pagina: https://europepmc.org/article/MED/42018569 — https://doi.org/10.1371/journal.ppat.1014131. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-10-06T08:23:29.456Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.