Metraipolis › archive › dc_bec404c8d47c

High-throughput, single-copy sequencing reveals SARS-CoV-2 spike variants coincident with mounting humoral immunity during acute COVID-19 — Europe PMC PMID:33831133

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/33831133
DOI: 10.1371/journal.ppat.1009431
License (as archived): CC0-1.0
Archived: 2026-11-05T01:35:33.909Z
SHA-256 of the archived text: 81211e340f1885d0a68011d773bea8f7387175b6480785a5dd7144ce382d7150
Stable URL of this page: https://metraipolis.com/documento/dc_bec404c8d47c

How to cite

High-throughput, single-copy sequencing reveals SARS-CoV-2 spike variants coincident with mounting humoral immunity during acute COVID-19 — Europe PMC PMID:33831133 — Europe PMC (en), https://europepmc.org/article/MED/33831133 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_bec404c8d47c (Metraipolis archive dc_bec404c8d47c, sha256 81211e340f1885d0a68011d773bea8f7387175b6480785a5dd7144ce382d7150)

BibTeX

@misc{dcbec404c8d47c,
  title = {High-throughput, single-copy sequencing reveals SARS-CoV-2 spike variants coincident with mounting humoral immunity during acute COVID-19 — Europe PMC PMID:33831133},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/33831133},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_bec404c8d47c; sha256 81211e340f1885d0a68011d773bea8f7387175b6480785a5dd7144ce382d7150},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/33831133. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Tracking evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within infected individuals will help elucidate coronavirus disease 2019 (COVID-19) pathogenesis and inform use of antiviral interventions. In this study, we developed an approach for sequencing the region encoding the SARS-CoV-2 virion surface proteins from large numbers of individual virus RNA genomes per sample. We applied this approach to the WA-1 reference clinical isolate of SARS-CoV-2 passaged in vitro and to upper respiratory samples from 7 study participants with COVID-19. SARS-CoV-2 genomes from cell culture were diverse, including 18 haplotypes with non-synonymous mutations clustered in the spike NH2-terminal domain (NTD) and furin cleavage site regions. By contrast, cross-sectional analysis of samples from participants with COVID-19 showed fewer virus variants, without structural clustering of mutations. However, longitudinal analysis in one individual revealed 4 virus haplotypes bearing 3 independent mutations in a spike NTD epitope targeted by autologous antibodies. These mutations arose coincident with a 6.2-fold rise in serum binding to spike and a transient increase in virus burden. We conclude that SARS-CoV-2 exhibits a capacity for rapid genetic adaptation that becomes detectable in vivo with the onset of humoral immunity, with the potential to contribute to delayed virologic clearance in the acute setting.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PLoS pathogens, 2021 (prima pubblicazione 2021-04-08).
Identificativi: PMID 33831133 · PMCID PMC8031304 · DOI 10.1371/journal.ppat.1009431.
Descrittori MeSH principali: Epitopes; Mutation; Immunity, Humoral; Spike Glycoprotein, Coronavirus; COVID-19; SARS-CoV-2.
Pagina: https://europepmc.org/article/MED/33831133 — https://doi.org/10.1371/journal.ppat.1009431. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-11-05T01:28:58.736Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.