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Antibody repertoire induced by SARS-CoV-2 spike protein immunogens — Europe PMC PPR:PPR162421

Source: Europe PMC
Original: https://europepmc.org/article/PPR/PPR162421
DOI: 10.1101/2020.05.12.091918
License (as archived): CC0-1.0
Archived: 2026-11-08T17:23:19.684Z
SHA-256 of the archived text: 9ac55f0fca7a5ac88f5a64b77a721583288004055d511a419fac9973291669d4
Stable URL of this page: https://metraipolis.com/documento/dc_bd4eb3ebd703

How to cite

Antibody repertoire induced by SARS-CoV-2 spike protein immunogens — Europe PMC PPR:PPR162421 — Europe PMC, https://europepmc.org/article/PPR/PPR162421 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_bd4eb3ebd703 (Metraipolis archive dc_bd4eb3ebd703, sha256 9ac55f0fca7a5ac88f5a64b77a721583288004055d511a419fac9973291669d4)

BibTeX

@misc{dcbd4eb3ebd703,
  title = {Antibody repertoire induced by SARS-CoV-2 spike protein immunogens — Europe PMC PPR:PPR162421},
  howpublished = {Europe PMC},
  url = {https://europepmc.org/article/PPR/PPR162421},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_bd4eb3ebd703; sha256 9ac55f0fca7a5ac88f5a64b77a721583288004055d511a419fac9973291669d4},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC, https://europepmc.org/article/PPR/PPR162421. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

ABSTRACT: Multiple vaccine candidates against SARS-CoV-2 based on viral spike protein are under development. However, there is limited information on the quality of antibody response generated following vaccination by these vaccine modalities. To better understand antibody response induced by spike protein-based vaccines, we immunized rabbits with various SARS-CoV-2 spike protein antigens: S-ectodomain (S1+S2) (aa 16-1213), which lacks the cytoplasmic and transmembrane domains (CT-TM), the S1 domain (aa 16-685), the receptor-binding domain (RBD) (aa 319-541), and the S2 domain (aa 686-1213 as control). Antibody response was analyzed by ELISA, Surface Plasmon Resonance (SPR) against different Spike proteins in native conformation, and a pseudovirion neutralization assay to measure the quality and function of the antibodies elicited by the different Spike antigens. All three antigens (S1+S2 ectodomain, S1 domain, and RBD) generated strong neutralizing antibodies against SARS-CoV-2. Vaccination induced antibody repertoire was analyzed by SARS-CoV-2 spike Genome Fragment Phage Display Libraries (SARS-CoV-2 GFPDL), which identified immunodominant epitopes in the S1, S1-RBD and S2 domains. Furthermore, these analyses demonstrated that surprisingly the RBD immunogen elicited a higher antibody titer with 5-fold higher affinity antibodies to native spike antigens compared with other spike antigens. These findings may help guide rational vaccine design and facilitate development and evaluation of effective therapeutics and vaccines against COVID-19 disease. One Sentence Summary: SARS-CoV-2 Spike induced immune response

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: bioRxiv, 2020 (prima pubblicazione 2020-05-13).
Identificativi: DOI 10.1101/2020.05.12.091918.
Pagina: https://europepmc.org/article/PPR/PPR162421 — https://doi.org/10.1101/2020.05.12.091918. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-11-08T17:19:10.429Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.