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Structurally related but genetically unrelated antibody lineages converge on an immunodominant HIV-1 Env neutralizing determinant following trimer immunization — Europe PMC PMID:34559844

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/34559844
DOI: 10.1371/journal.ppat.1009543
License (as archived): CC0-1.0
Archived: 2026-11-02T17:42:19.609Z
SHA-256 of the archived text: acb4b829f961515aba4acbfb4a11e66e3aadfbafa15ae8c183e6f3b0eef40e6c
Stable URL of this page: https://metraipolis.com/documento/dc_abc347cd5abd

How to cite

Structurally related but genetically unrelated antibody lineages converge on an immunodominant HIV-1 Env neutralizing determinant following trimer immunization — Europe PMC PMID:34559844 — Europe PMC (en), https://europepmc.org/article/MED/34559844 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_abc347cd5abd (Metraipolis archive dc_abc347cd5abd, sha256 acb4b829f961515aba4acbfb4a11e66e3aadfbafa15ae8c183e6f3b0eef40e6c)

BibTeX

@misc{dcabc347cd5abd,
  title = {Structurally related but genetically unrelated antibody lineages converge on an immunodominant HIV-1 Env neutralizing determinant following trimer immunization — Europe PMC PMID:34559844},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/34559844},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_abc347cd5abd; sha256 acb4b829f961515aba4acbfb4a11e66e3aadfbafa15ae8c183e6f3b0eef40e6c},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/34559844. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Understanding the molecular mechanisms by which antibodies target and neutralize the HIV-1 envelope glycoprotein (Env) is critical in guiding immunogen design and vaccine development aimed at eliciting cross-reactive neutralizing antibodies (NAbs). Here, we analyzed monoclonal antibodies (mAbs) isolated from non-human primates (NHPs) immunized with variants of a native flexibly linked (NFL) HIV-1 Env stabilized trimer derived from the tier 2 clade C 16055 strain. The antibodies displayed neutralizing activity against the autologous virus with potencies ranging from 0.005 to 3.68 μg/ml (IC50). Structural characterization using negative-stain EM and X-ray crystallography identified the variable region 2 (V2) of the 16055 NFL trimer to be the common epitope for these antibodies. The crystal structures revealed that the V2 segment adopts a β-hairpin motif identical to that observed in the 16055 NFL crystal structure. These results depict how vaccine-induced antibodies derived from different clonal lineages penetrate through the glycan shield to recognize a hypervariable region within V2 (residues 184-186) that is unique to the 16055 strain. They also provide potential explanations for the potent autologous neutralization of these antibodies, confirming the immunodominance of this site and revealing that multiple angles of approach are permissible for affinity/avidity that results in potent neutralizing capacity. The structural analysis reveals that the most negatively charged paratope correlated with the potency of the mAbs. The atomic level information is of interest to both define the means of autologous neutralization elicited by different tier 2-based immunogens and facilitate trimer redesign to better target more conserved regions of V2 to potentially elicit cross-neutralizing HIV-1 antibodies.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PLoS pathogens, 2021 (prima pubblicazione 2021-09-24).
Identificativi: PMID 34559844 · PMCID PMC8494329 · DOI 10.1371/journal.ppat.1009543.
Pagina: https://europepmc.org/article/MED/34559844 — https://doi.org/10.1371/journal.ppat.1009543. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
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