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Metabolically active bacteria detected with click chemistry in low organic matter rainwater — Europe PMC PMID:37200308

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/37200308
DOI: 10.1371/journal.pone.0285816
License (as archived): CC0-1.0
Archived: 2026-10-07T13:26:43.807Z
SHA-256 of the archived text: 810629f18eb274c7b89113398d38449404e0ec1a6a9ec0a45d3b0275575c82cb
Stable URL of this page: https://metraipolis.com/documento/dc_9d89899bc392

How to cite

Metabolically active bacteria detected with click chemistry in low organic matter rainwater — Europe PMC PMID:37200308 — Europe PMC (en), https://europepmc.org/article/MED/37200308 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_9d89899bc392 (Metraipolis archive dc_9d89899bc392, sha256 810629f18eb274c7b89113398d38449404e0ec1a6a9ec0a45d3b0275575c82cb)

BibTeX

@misc{dc9d89899bc392,
  title = {Metabolically active bacteria detected with click chemistry in low organic matter rainwater — Europe PMC PMID:37200308},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/37200308},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_9d89899bc392; sha256 810629f18eb274c7b89113398d38449404e0ec1a6a9ec0a45d3b0275575c82cb},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/37200308. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Rain contains encapsulated bacteria that can be transported over vast distances during relatively short periods of time. However, the ecological significance of bacteria in "precontact" rainwater-rainwater prior to contact with non-atmospheric surfaces-remains relatively undefined given the methodological challenges of studying low-abundance microbes in a natural assemblage. Here, we implement single-cell "click" chemistry in a novel application to detect the protein synthesis of bacteria in precontact rainwater samples as a measure of metabolic activity. Using epifluorescence microscopy, we find approximately 103-104 bacteria cells mL-1 with up to 7.2% of the observed cells actively synthesizing protein. Additionally, our measurement of less than 30 μM total organic carbon in the samples show that some rainwater bacteria can metabolize substrates in very low organic matter conditions, comparable to extremophiles in the deep ocean. Overall, our results raise new questions for the field of rainwater microbiology and may help inform efforts to develop quantitative microbial risk assessments for the appropriate use of harvested rainwater.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PloS one, 2023 (prima pubblicazione 2023-05-18).
Identificativi: PMID 37200308 · PMCID PMC10194877 · DOI 10.1371/journal.pone.0285816.
Descrittori MeSH principali: Water Microbiology; Click Chemistry.
Pagina: https://europepmc.org/article/MED/37200308 — https://doi.org/10.1371/journal.pone.0285816. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-10-07T12:57:59.535Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.