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Tamoxifen-induced gene expression — Wikipedia (en)

Source: Wikipedia (en)
Original: https://en.wikipedia.org/wiki/Tamoxifen-induced_gene_expression
License (as archived): CC-BY-SA-4.0
Archived: 2026-10-07T19:02:42.092Z
SHA-256 of the archived text: 705f7cb1915407c663651398236c8604157b359f14aabdab8ae2a7535799ceba
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Tamoxifen-induced gene expression — Wikipedia (en) — Wikipedia (en), https://en.wikipedia.org/wiki/Tamoxifen-induced_gene_expression (CC-BY-SA-4.0); archived copy: https://metraipolis.com/documento/dc_8e30fa6d283f (Metraipolis archive dc_8e30fa6d283f, sha256 705f7cb1915407c663651398236c8604157b359f14aabdab8ae2a7535799ceba)

BibTeX

@misc{dc8e30fa6d283f,
  title = {Tamoxifen-induced gene expression — Wikipedia (en)},
  howpublished = {Wikipedia (en)},
  url = {https://en.wikipedia.org/wiki/Tamoxifen-induced_gene_expression},
  note = {License CC-BY-SA-4.0; archived copy https://metraipolis.com/documento/dc_8e30fa6d283f; sha256 705f7cb1915407c663651398236c8604157b359f14aabdab8ae2a7535799ceba},
  year = {2026}
}

Archived text

Reproduced under CC-BY-SA-4.0. Attribution: Wikipedia (en), https://en.wikipedia.org/wiki/Tamoxifen-induced_gene_expression. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Tamoxifen-induced gene expression is a method used in molecular biology and neuroscience to activate transcription of a specific gene by application of the drug tamoxifen. It is based on the expression of a mutated estrogen receptor (ERT2) fused to Cre recombinase, an inducible gene expression system. In the absence of tamoxifen, the receptor is inactive and sequestered in the cytoplasm. When tamoxifen is administered, it binds to ERT2, causing the Cre-ERT2 fusion protein to move into the cell's nucleus, where it can perform its recombination function. The gene of interest, encoding e.g. the fluorescent protein GFP, contains a LoxP-flanked stop cassette that prevents expression unless it is excised by Cre recombinase. When used in mice, the Cre-ERT2 system allows for excellent temporal control: Administration of tamoxifen triggers genetic changes at a precise time point in development or adult life. However, depending on the type of target cell, the gene of interest may not be successfully activated in every cell, leading to mosaic expression.

Tema degli esploratori: scienze della vita e salute (deposito sistematico; categoria «Neuroscience»).
Fonte: Wikipedia in inglese, voce «Tamoxifen-induced gene expression» — https://en.wikipedia.org/wiki/Tamoxifen-induced_gene_expression
Wikidata Q137799595 (dati Wikidata in CC0 1.0) — https://www.wikidata.org/wiki/Q137799595
Autori: i contributori di Wikipedia (cronologia: https://en.wikipedia.org/wiki/Tamoxifen-induced_gene_expression?action=history).
Licenza: Creative Commons Attribuzione-Condividi allo stesso modo 4.0 (CC BY-SA 4.0) — https://creativecommons.org/licenses/by-sa/4.0/. Il riuso richiede attribuzione e stessa licenza.
Estratto dell'introduzione non modificato nel contenuto; revisione 1378184769, consultata il 2026-10-07T18:25:37.068Z tramite l'API ufficiale di Wikipedia. Impronta SHA-256 dell'estratto: ea09a19aeb7c7ac06c57c944b70d8f0caba9afa67011653944e192e94aff0705