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Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage — Europe PMC PMID:38507462

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/38507462
DOI: 10.7554/elife.86764
License (as archived): CC0-1.0
Archived: 2026-10-06T20:06:39.118Z
SHA-256 of the archived text: 63b48e2429c9d6acdefae67d821fd9430ef0233664b4ed6698df75279d6882d3
Stable URL of this page: https://metraipolis.com/documento/dc_7ed855be38e2

How to cite

Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage — Europe PMC PMID:38507462 — Europe PMC (en), https://europepmc.org/article/MED/38507462 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_7ed855be38e2 (Metraipolis archive dc_7ed855be38e2, sha256 63b48e2429c9d6acdefae67d821fd9430ef0233664b4ed6698df75279d6882d3)

BibTeX

@misc{dc7ed855be38e2,
  title = {Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage — Europe PMC PMID:38507462},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/38507462},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_7ed855be38e2; sha256 63b48e2429c9d6acdefae67d821fd9430ef0233664b4ed6698df75279d6882d3},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/38507462. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

The trimeric SARS-CoV-2 Spike protein mediates viral attachment facilitating cell entry. Most COVID-19 vaccines direct mammalian cells to express the Spike protein or deliver it directly via inoculation to engender a protective immune response. The trafficking and cellular tropism of the Spike protein in vivo and its impact on immune cells remains incompletely elucidated. In this study, we inoculated mice intranasally, intravenously, and subcutaneously with fluorescently labeled recombinant SARS-CoV-2 Spike protein. Using flow cytometry and imaging techniques, we analyzed its localization, immune cell tropism, and acute functional impact. Intranasal administration led to rapid lung alveolar macrophage uptake, pulmonary vascular leakage, and neutrophil recruitment and damage. When injected near the inguinal lymph node medullary, but not subcapsular macrophages, captured the protein, while scrotal injection recruited and fragmented neutrophils. Widespread endothelial and liver Kupffer cell uptake followed intravenous administration. Human peripheral blood cells B cells, neutrophils, monocytes, and myeloid dendritic cells all efficiently bound Spike protein. Exposure to the Spike protein enhanced neutrophil NETosis and augmented human macrophage TNF-α (tumor necrosis factor-α) and IL-6 production. Human and murine immune cells employed C-type lectin receptors and Siglecs to help capture the Spike protein. This study highlights the potential toxicity of the SARS-CoV-2 Spike protein for mammalian cells and illustrates the central role for alveolar macrophage in pathogenic protein uptake.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: eLife, 2024 (prima pubblicazione 2024-03-20).
Identificativi: PMID 38507462 · PMCID PMC10954308 · DOI 10.7554/elife.86764.
Parole chiave degli autori: Mouse; PBMC; Spike protein; Inflammation; Viral vector; Alveolar macrophage; neutrophil; immunology; Netosis; Siglec; Sars-cov-2.
Descrittori MeSH principali: Spike Glycoprotein, Coronavirus; COVID-19.
Pagina: https://europepmc.org/article/MED/38507462 — https://doi.org/10.7554/elife.86764. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-10-06T18:30:56.465Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.