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Sodium butyrate modulates chicken macrophage proteins essential for Salmonella Enteritidis invasion — Europe PMC PMID:33909627

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/33909627
DOI: 10.1371/journal.pone.0250296
License (as archived): CC0-1.0
Archived: 2026-11-04T21:44:20.846Z
SHA-256 of the archived text: db7d1d44fd075d0a4ee900ab6067f220362303727bd8a676b57d7cb3f80abf56
Stable URL of this page: https://metraipolis.com/documento/dc_72d4ae2c679a

How to cite

Sodium butyrate modulates chicken macrophage proteins essential for Salmonella Enteritidis invasion — Europe PMC PMID:33909627 — Europe PMC (en), https://europepmc.org/article/MED/33909627 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_72d4ae2c679a (Metraipolis archive dc_72d4ae2c679a, sha256 db7d1d44fd075d0a4ee900ab6067f220362303727bd8a676b57d7cb3f80abf56)

BibTeX

@misc{dc72d4ae2c679a,
  title = {Sodium butyrate modulates chicken macrophage proteins essential for Salmonella Enteritidis invasion — Europe PMC PMID:33909627},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/33909627},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_72d4ae2c679a; sha256 db7d1d44fd075d0a4ee900ab6067f220362303727bd8a676b57d7cb3f80abf56},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/33909627. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Salmonella Enteritidis is an intracellular foodborne pathogen that has developed multiple mechanisms to alter poultry intestinal physiology and infect the gut. Short chain fatty acid butyrate is derived from microbiota metabolic activities, and it maintains gut homeostasis. There is limited understanding on the interaction between S. Enteritidis infection, butyrate, and host intestinal response. To fill this knowledge gap, chicken macrophages (also known as HTC cells) were infected with S. Enteritidis, treated with sodium butyrate, and proteomic analysis was performed. A growth curve assay was conducted to determine sub-inhibitory concentration (SIC, concentration that do not affect bacterial growth compared to control) of sodium butyrate against S. Enteritidis. HTC cells were infected with S. Enteritidis in the presence and absence of SIC of sodium butyrate. The proteins were extracted and analyzed by tandem mass spectrometry. Our results showed that the SIC was 45 mM. Notably, S. Enteritidis-infected HTC cells upregulated macrophage proteins involved in ATP synthesis through oxidative phosphorylation such as ATP synthase subunit alpha (ATP5A1), ATP synthase subunit d, mitochondrial (ATP5PD) and cellular apoptosis such as Cytochrome-c (CYC). Furthermore, sodium butyrate influenced S. Enteritidis-infected HTC cells by reducing the expression of macrophage proteins mediating actin cytoskeletal rearrangements such as WD repeat-containing protein-1 (WDR1), Alpha actinin-1 (ACTN1), Vinculin (VCL) and Protein disulfide isomerase (P4HB) and intracellular S. Enteritidis growth and replication such as V-type proton ATPase catalytic subunit A (ATPV1A). Interestingly, sodium butyrate increased the expression of infected HTC cell protein involving in bacterial killing such as Vimentin (VIM). In conclusion, sodium butyrate modulates the expression of HTC cell proteins essential for S. Enteritidis invasion.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PloS one, 2021 (prima pubblicazione 2021-04-28).
Identificativi: PMID 33909627 · PMCID PMC8081216 · DOI 10.1371/journal.pone.0250296.
Pagina: https://europepmc.org/article/MED/33909627 — https://doi.org/10.1371/journal.pone.0250296. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-11-04T21:36:15.858Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.