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Tailored design of protein nanoparticle scaffolds for multivalent presentation of viral glycoprotein antigens — Europe PMC PMID:32748788

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/32748788
DOI: 10.7554/elife.57659
License (as archived): CC0-1.0
Archived: 2026-11-07T21:09:29.209Z
SHA-256 of the archived text: d3a5c8bf79b14cf16e78bdbd867306656b0eb279132cb57c05d6d59a241a3b41
Stable URL of this page: https://metraipolis.com/documento/dc_608ae80edd40

How to cite

Tailored design of protein nanoparticle scaffolds for multivalent presentation of viral glycoprotein antigens — Europe PMC PMID:32748788 — Europe PMC (en), https://europepmc.org/article/MED/32748788 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_608ae80edd40 (Metraipolis archive dc_608ae80edd40, sha256 d3a5c8bf79b14cf16e78bdbd867306656b0eb279132cb57c05d6d59a241a3b41)

BibTeX

@misc{dc608ae80edd40,
  title = {Tailored design of protein nanoparticle scaffolds for multivalent presentation of viral glycoprotein antigens — Europe PMC PMID:32748788},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/32748788},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_608ae80edd40; sha256 d3a5c8bf79b14cf16e78bdbd867306656b0eb279132cb57c05d6d59a241a3b41},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/32748788. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Multivalent presentation of viral glycoproteins can substantially increase the elicitation of antigen-specific antibodies. To enable a new generation of anti-viral vaccines, we designed self-assembling protein nanoparticles with geometries tailored to present the ectodomains of influenza, HIV, and RSV viral glycoprotein trimers. We first de novo designed trimers tailored for antigen fusion, featuring N-terminal helices positioned to match the C termini of the viral glycoproteins. Trimers that experimentally adopted their designed configurations were incorporated as components of tetrahedral, octahedral, and icosahedral nanoparticles, which were characterized by cryo-electron microscopy and assessed for their ability to present viral glycoproteins. Electron microscopy and antibody binding experiments demonstrated that the designed nanoparticles presented antigenically intact prefusion HIV-1 Env, influenza hemagglutinin, and RSV F trimers in the predicted geometries. This work demonstrates that antigen-displaying protein nanoparticles can be designed from scratch, and provides a systematic way to investigate the influence of antigen presentation geometry on the immune response to vaccination.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: eLife, 2020 (prima pubblicazione 2020-08-04).
Identificativi: PMID 32748788 · PMCID PMC7402677 · DOI 10.7554/elife.57659.
Parole chiave degli autori: Human; B lymphocytes; Virus; E. coli; Inflammation; systems biology; computational biology; immunology; Hek293f; Bl21; Lemo21.
Descrittori MeSH principali: Immunity, Humoral.
Pagina: https://europepmc.org/article/MED/32748788 — https://doi.org/10.7554/elife.57659. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-11-07T21:05:21.715Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.