Metraipolis › archive › dc_5b694c4c1927

Carbohydrate antigen microarray analysis of serum IgG and IgM antibodies before and after adult porcine islet xenotransplantation in cynomolgus macaques — Europe PMC PMID:34138941

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/34138941
DOI: 10.1371/journal.pone.0253029
License (as archived): CC0-1.0
Archived: 2026-11-04T01:31:04.215Z
SHA-256 of the archived text: 0ca3a787180e9897a1c7af72148cdd260b05cd9a696fe6e1b8baebad388ce033
Stable URL of this page: https://metraipolis.com/documento/dc_5b694c4c1927

How to cite

Carbohydrate antigen microarray analysis of serum IgG and IgM antibodies before and after adult porcine islet xenotransplantation in cynomolgus macaques — Europe PMC PMID:34138941 — Europe PMC (en), https://europepmc.org/article/MED/34138941 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_5b694c4c1927 (Metraipolis archive dc_5b694c4c1927, sha256 0ca3a787180e9897a1c7af72148cdd260b05cd9a696fe6e1b8baebad388ce033)

BibTeX

@misc{dc5b694c4c1927,
  title = {Carbohydrate antigen microarray analysis of serum IgG and IgM antibodies before and after adult porcine islet xenotransplantation in cynomolgus macaques — Europe PMC PMID:34138941},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/34138941},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_5b694c4c1927; sha256 0ca3a787180e9897a1c7af72148cdd260b05cd9a696fe6e1b8baebad388ce033},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/34138941. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Understanding the anti-carbohydrate antibody response toward epitopes expressed on porcine cells, tissues, and organs is critical to advancing xenotransplantation toward clinical application. In this study, we determined IgM and IgG antibody specificities and relative concentrations in five cynomolgus monkeys at baseline and at intervals following intraportal xenotransplantation of adult porcine islets. This study utilized a carbohydrate antigen microarray that comprised more than 400 glycoconjugates, including historically reported α-Gal and non-α-Gal carbohydrate antigens with various modifications. The elicited anti-carbohydrate antibody responses were predominantly IgM compared to IgG in 4 out of 5 monkeys. Patterns of elicited antibody responses greater than 1.5 difference (log2 base units; 2.8-fold on a linear scale) from pre-serum to post-serum sampling specific for carbohydrate antigens were heterogeneous and recipient-specific. Increases in the elicited antibody response to α-Gal, Sda, GM2 antigens, or Lexis X antigen were found in individual monkeys. The novel carbohydrate structures Galβ1-4GlcNAcβ1-3Galβ1 and N-linked glycans with Manα1-6(GlcNAcβ1-2Manα1-3)Manβ1-4GlcNAcβ structure were common targets of elicited IgM antibodies. These results provide important insights into the carbohydrate epitopes that elicit antibodies following pig-to-monkey islet xenotransplantation and reveal possible targets for gene editing.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PloS one, 2021 (prima pubblicazione 2021-06-17).
Identificativi: PMID 34138941 · PMCID PMC8211184 · DOI 10.1371/journal.pone.0253029.
Pagina: https://europepmc.org/article/MED/34138941 — https://doi.org/10.1371/journal.pone.0253029. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-11-04T01:22:45.622Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.