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Evaluation of Lipids and Lipid-Related Transcripts in Human and Ovine Theca Cells and an in Vitro Mouse Model Exposed to the Obesogen Chemical Tributyltin — Europe PMC PMID:38630605

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/38630605
DOI: 10.1289/ehp13955
License (as archived): CC0-1.0
Archived: 2026-10-06T18:47:39.154Z
SHA-256 of the archived text: 150248b7d58e2712d560e34f5b6ddee94ddbf80da7017222a3812dba2a2906be
Stable URL of this page: https://metraipolis.com/documento/dc_59faaba3cf30

How to cite

Evaluation of Lipids and Lipid-Related Transcripts in Human and Ovine Theca Cells and an in Vitro Mouse Model Exposed to the Obesogen Chemical Tributyltin — Europe PMC PMID:38630605 — Europe PMC (en), https://europepmc.org/article/MED/38630605 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_59faaba3cf30 (Metraipolis archive dc_59faaba3cf30, sha256 150248b7d58e2712d560e34f5b6ddee94ddbf80da7017222a3812dba2a2906be)

BibTeX

@misc{dc59faaba3cf30,
  title = {Evaluation of Lipids and Lipid-Related Transcripts in Human and Ovine Theca Cells and an in Vitro Mouse Model Exposed to the Obesogen Chemical Tributyltin — Europe PMC PMID:38630605},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/38630605},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_59faaba3cf30; sha256 150248b7d58e2712d560e34f5b6ddee94ddbf80da7017222a3812dba2a2906be},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/38630605. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Background: Exposure to obesogenic chemicals has been reported to result in enhanced adipogenesis, higher adipose tissue accumulation, and reduced ovarian hormonal synthesis and follicular function. We have reported that organotins [tributyltin (TBT) and triphenyltin (TPT)] dysregulate cholesterol trafficking in ovarian theca cells, but, whether organotins also exert lipogenic effects on ovarian cells remains unexplored. Objective: We investigated if environmentally relevant exposures to organotins [TBT, TPT, or dibutyltin (DBT)] induce lipid dysregulation in ovarian theca cells and the role of the liver X receptor (LXR) in this effect. We also tested the effect of TBT on oocyte maturation and neutral lipid accumulation, and lipid-related transcript expression in cumulus cells and preimplantation embryos. Methods: Primary theca cell cultures derived from human and ovine ovaries were exposed to TBT, TPT, or DBT (1, 10, or 50 ng/ml). The effect of these chemical exposures on neutral lipid accumulation, lipid abundance and composition, lipid homeostasis-related gene expression, and cytokine secretion was evaluated using liquid chromatography-mass spectrometry (LC-MS), inhibitor-based methods, cytokine secretion, and lipid ontology analyses. We also exposed murine cumulus-oocyte complexes to TBT and evaluated oocyte maturation, embryo development, and lipid homeostasis-related mRNA expression in cumulus cells and blastocysts. Results: Exposure to TBT resulted in higher intracellular neutral lipids in human and ovine primary theca cells. In ovine theca cells, this effect was dose-dependent, independent of cell stage, and partially mediated by LXR. DBT and TPT resulted in higher intracellular neutral lipids but to a lesser extent in comparison with TBT. More than 140 lipids and 9 cytokines were dysregulated in TBT-exposed human theca cells. Expression of genes involved in lipogenesis and fatty acid synthesis were higher in theca cells, as well as in cumulus cells and blastocysts exposed to TBT. However, TBT did not impact the rates of oocyte maturation or blastocyst development. Discussion: TBT induced dyslipidemia in primary human and ovine theca cells, which may be responsible for some of the TBT-induced fertility dysregulations reported in rodent models of TBT exposure. https://doi.org/10.1289/EHP13955.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: Environmental health perspectives, 2024 (prima pubblicazione 2024-04-17).
Identificativi: PMID 38630605 · PMCID PMC11023052 · DOI 10.1289/ehp13955.
Descrittori MeSH principali: Theca Cells; Organotin Compounds; Trialkyltin Compounds.
Pagina: https://europepmc.org/article/MED/38630605 — https://doi.org/10.1289/ehp13955. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-10-06T17:12:53.319Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.