Metraipolis › archive › dc_49e579ffbb2c

Viral proteogenomic and expression profiling during productive replication of a skin-tropic herpesvirus in the natural host — Europe PMC PMID:37289833

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/37289833
DOI: 10.1371/journal.ppat.1011204
License (as archived): CC0-1.0
Archived: 2026-10-07T12:59:30.598Z
SHA-256 of the archived text: cb929316a6b8d0f6ca066d282e4ece6a3c3dd0160604cd6b0e2e9effdd87fa1d
Stable URL of this page: https://metraipolis.com/documento/dc_49e579ffbb2c

How to cite

Viral proteogenomic and expression profiling during productive replication of a skin-tropic herpesvirus in the natural host — Europe PMC PMID:37289833 — Europe PMC (en), https://europepmc.org/article/MED/37289833 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_49e579ffbb2c (Metraipolis archive dc_49e579ffbb2c, sha256 cb929316a6b8d0f6ca066d282e4ece6a3c3dd0160604cd6b0e2e9effdd87fa1d)

BibTeX

@misc{dc49e579ffbb2c,
  title = {Viral proteogenomic and expression profiling during productive replication of a skin-tropic herpesvirus in the natural host — Europe PMC PMID:37289833},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/37289833},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_49e579ffbb2c; sha256 cb929316a6b8d0f6ca066d282e4ece6a3c3dd0160604cd6b0e2e9effdd87fa1d},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/37289833. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Efficient transmission of herpesviruses is essential for dissemination in host populations; however, little is known about the viral genes that mediate transmission, mostly due to a lack of natural virus-host model systems. Marek's disease is a devastating herpesviral disease of chickens caused by Marek's disease virus (MDV) and an excellent natural model to study skin-tropic herpesviruses and transmission. Like varicella zoster virus that causes chicken pox in humans, the only site where infectious cell-free MD virions are efficiently produced is in epithelial skin cells, a requirement for host-to-host transmission. Here, we enriched for heavily infected feather follicle epithelial skin cells of live chickens to measure both viral transcription and protein expression using combined short- and long-read RNA sequencing and LC/MS-MS bottom-up proteomics. Enrichment produced a previously unseen breadth and depth of viral peptide sequencing. We confirmed protein translation for 84 viral genes at high confidence (1% FDR) and correlated relative protein abundance with RNA expression levels. Using a proteogenomic approach, we confirmed translation of most well-characterized spliced viral transcripts and identified a novel, abundant isoform of the 14 kDa transcript family via IsoSeq transcripts, short-read intron-spanning sequencing reads, and a high-quality junction-spanning peptide identification. We identified peptides representing alternative start codon usage in several genes and putative novel microORFs at the 5' ends of two core herpesviral genes, pUL47 and ICP4, along with strong evidence of independent transcription and translation of the capsid scaffold protein pUL26.5. Using a natural animal host model system to examine viral gene expression provides a robust, efficient, and meaningful way of validating results gathered from cell culture systems.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PLoS pathogens, 2023 (prima pubblicazione 2023-06-08).
Identificativi: PMID 37289833 · PMCID PMC10284419 · DOI 10.1371/journal.ppat.1011204.
Descrittori MeSH principali: Herpesviridae; Herpesvirus 2, Gallid; Marek Disease; Proteogenomics.
Pagina: https://europepmc.org/article/MED/37289833 — https://doi.org/10.1371/journal.ppat.1011204. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-10-07T12:29:42.045Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.