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Structural characterization and dynamics of AdhE ultrastructures from Clostridium thermocellum show a containment strategy for toxic intermediates — Europe PMC PMID:40577193

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/40577193
DOI: 10.7554/elife.96966
License (as archived): CC0-1.0
Archived: 2026-10-06T09:50:11.469Z
SHA-256 of the archived text: 950c7b0193a8122f0f6afb59dbfafc6ec6b97dc440129e94bab5e62526d3411c
Stable URL of this page: https://metraipolis.com/documento/dc_3d64cb347bce

How to cite

Structural characterization and dynamics of AdhE ultrastructures from Clostridium thermocellum show a containment strategy for toxic intermediates — Europe PMC PMID:40577193 — Europe PMC (en), https://europepmc.org/article/MED/40577193 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_3d64cb347bce (Metraipolis archive dc_3d64cb347bce, sha256 950c7b0193a8122f0f6afb59dbfafc6ec6b97dc440129e94bab5e62526d3411c)

BibTeX

@misc{dc3d64cb347bce,
  title = {Structural characterization and dynamics of AdhE ultrastructures from Clostridium thermocellum show a containment strategy for toxic intermediates — Europe PMC PMID:40577193},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/40577193},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_3d64cb347bce; sha256 950c7b0193a8122f0f6afb59dbfafc6ec6b97dc440129e94bab5e62526d3411c},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/40577193. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Clostridium thermocellum , a cellulolytic thermophilic anaerobe, is considered by many to be a prime candidate for the realization of consolidated bioprocessing (CBP) and is known as an industry standard for biofuel production. C. thermocellum is among the best biomass degraders identified to date in nature and produces ethanol as one of its main products. Many studies have helped increase ethanol titers in this microbe; however, ethanol production using C. thermocellum is still not economically viable. Therefore, a better understanding of its ethanol synthesis pathway is required. The main pathway for ethanol production in C. thermocellum involves the bifunctional aldehyde-alcohol dehydrogenase (AdhE). To better understand the function of the C. thermocellum AdhE, we used cryo-electron microscopy (cryo-EM) to obtain a 3.28 Å structure of the AdhE complex. This high-resolution structure, in combination with molecular dynamics simulations, provides insight into the substrate channeling of the toxic intermediate acetaldehyde, indicates the potential role of C. thermocellum AdhE to regulate activity and cofactor pools, and establishes a basis for future engineering studies. The containment strategy found in this enzyme offers a template that could be replicated in other systems where toxic intermediates need to be sequestered to increase the production of valuable biochemicals.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: eLife, 2025 (prima pubblicazione 2025-06-27).
Identificativi: PMID 40577193 · PMCID PMC12204686 · DOI 10.7554/elife.96966.
Parole chiave degli autori: E. coli; systems biology; computational biology; Thermophiles; Structural Biology; Cofactors; Cryo-em; Substrate Channeling; C. Thermocellum; Molecular Biophysics; Aldehyde-alcohol Dehydrogenase.
Descrittori MeSH principali: Ethanol; Alcohol Dehydrogenase; Bacterial Proteins; Acetivibrio thermocellus.
Pagina: https://europepmc.org/article/MED/40577193 — https://doi.org/10.7554/elife.96966. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-10-06T09:43:37.993Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.