Metraipolis › archive › dc_3c1904b603b8

Interphase-arrested Drosophila embryos activate zygotic gene expression and initiate mid-blastula transition events at a low nuclear-cytoplasmic ratio — Europe PMC PMID:33090988

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/33090988
DOI: 10.1371/journal.pbio.3000891
License (as archived): CC0-1.0
Archived: 2026-11-07T03:08:24.784Z
SHA-256 of the archived text: ae4d03a1b2093b4687cf578e6bfaaed14d4faf06ce761703933e472fca94a36e
Stable URL of this page: https://metraipolis.com/documento/dc_3c1904b603b8

How to cite

Interphase-arrested Drosophila embryos activate zygotic gene expression and initiate mid-blastula transition events at a low nuclear-cytoplasmic ratio — Europe PMC PMID:33090988 — Europe PMC (en), https://europepmc.org/article/MED/33090988 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_3c1904b603b8 (Metraipolis archive dc_3c1904b603b8, sha256 ae4d03a1b2093b4687cf578e6bfaaed14d4faf06ce761703933e472fca94a36e)

BibTeX

@misc{dc3c1904b603b8,
  title = {Interphase-arrested Drosophila embryos activate zygotic gene expression and initiate mid-blastula transition events at a low nuclear-cytoplasmic ratio — Europe PMC PMID:33090988},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/33090988},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_3c1904b603b8; sha256 ae4d03a1b2093b4687cf578e6bfaaed14d4faf06ce761703933e472fca94a36e},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/33090988. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Externally deposited eggs begin development with an immense cytoplasm and a single overwhelmed nucleus. Rapid mitotic cycles restore normality as the ratio of nuclei to cytoplasm (N/C) increases. A threshold N/C has been widely proposed to activate zygotic genome transcription and onset of morphogenesis at the mid-blastula transition (MBT). To test whether a threshold N/C is required for these events, we blocked N/C increase by down-regulating cyclin/Cdk1 to arrest early cell cycles in Drosophila. Embryos that were arrested two cell cycles prior to the normal MBT activated widespread transcription of the zygotic genome including genes previously described as N/C dependent. Zygotic transcription of these genes largely retained features of their regulation in space and time. Furthermore, zygotically regulated post-MBT events such as cellularization and gastrulation movements occurred in these cell cycle-arrested embryos. These results are not compatible with models suggesting that these MBT events are directly coupled to N/C. Cyclin/Cdk1 activity normally declines in tight association with increasing N/C and is regulated by N/C. By experimentally promoting the decrease in cyclin/Cdk1, we uncoupled MBT from N/C increase, arguing that N/C-guided down-regulation of cyclin/Cdk1 is sufficient for genome activation and MBT.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PLoS biology, 2020 (prima pubblicazione 2020-10-22).
Identificativi: PMID 33090988 · PMCID PMC7608951 · DOI 10.1371/journal.pbio.3000891.
Descrittori MeSH principali: Interphase; Gene Expression Regulation, Developmental; Cell Cycle Checkpoints.
Pagina: https://europepmc.org/article/MED/33090988 — https://doi.org/10.1371/journal.pbio.3000891. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-11-07T03:00:19.735Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.