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Procedure for spotted fever group Rickettsia isolation from limited clinical blood specimens — Europe PMC PMID:36240222

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/36240222
DOI: 10.1371/journal.pntd.0010781
License (as archived): CC0-1.0
Archived: 2026-10-16T06:40:32.020Z
SHA-256 of the archived text: 82630b8dfe2e12a3ab6b1385dd033ba98f80a4db8e8919ad92f4e1f47a884862
Stable URL of this page: https://metraipolis.com/documento/dc_3202976c9bbc

How to cite

Procedure for spotted fever group Rickettsia isolation from limited clinical blood specimens — Europe PMC PMID:36240222 — Europe PMC (en), https://europepmc.org/article/MED/36240222 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_3202976c9bbc (Metraipolis archive dc_3202976c9bbc, sha256 82630b8dfe2e12a3ab6b1385dd033ba98f80a4db8e8919ad92f4e1f47a884862)

BibTeX

@misc{dc3202976c9bbc,
  title = {Procedure for spotted fever group Rickettsia isolation from limited clinical blood specimens — Europe PMC PMID:36240222},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/36240222},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_3202976c9bbc; sha256 82630b8dfe2e12a3ab6b1385dd033ba98f80a4db8e8919ad92f4e1f47a884862},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/36240222. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Background: Current isolation techniques for spotted fever group Rickettsia from clinical samples are laborious and are limited to tissue, blood and blood derivatives with volumes ideally greater than 1 mL. We validated the use of simplified methodologies for spotted fever group Rickettsia culture isolation that overcome sample volume limitations and provide utility in clinical diagnostics and research studies. Methodology/principal findings: A modified cell culture method is evaluated for the isolation of Rickettsia ssp. from human diagnostic samples. Culture sampling method, culture platform, and growth phase analysis were evaluated to determine best practices for optimal culture isolation conditions. Rickettsial isolates (R. conorii, R. rickettsii, and R. parkeri) were grown in Vero E6 cells over a course of 5 to 7 days at low inoculum treatments (~40 bacterial copies) to standardize the sampling strategy at a copy number reflective of the bacteremia in acute diagnostic samples. This methodology was verified using small volumes (50 μL) of 25 unprocessed clinical whole blood, plasma, and serum samples from acute samples of patients suspected of having Rocky Mountain Spotted Fever, of which 10 were previously confirmed positive via the PanR8 qPCR assay, 13 had no detectable Rickettsia DNA by the PanR8 qPCR assay, and 2 were not previously tested; these samples resulted in the cultivation of 7 new R. rickettsii isolates. Conclusions/significance: We observed that rickettsial isolate growth in culture is reproducibly identified by real-time PCR testing of culture media within 72 hours after inoculation. Additionally, specimen sedimentation prior to isolation to remove red blood cells was found to decrease the amount of total organism available in the inoculum. A small volume culture method was established focusing on comparative qPCR detection rather than bacterial visualization, taking significantly shorter time to detect, and requiring less manipulation compared to traditional clinical isolate culture methods.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PLoS neglected tropical diseases, 2022 (prima pubblicazione 2022-10-14).
Identificativi: PMID 36240222 · PMCID PMC9605293 · DOI 10.1371/journal.pntd.0010781.
Descrittori MeSH principali: Rickettsia; Rocky Mountain Spotted Fever.
Pagina: https://europepmc.org/article/MED/36240222 — https://doi.org/10.1371/journal.pntd.0010781. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-10-16T06:20:18.909Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.