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Comparison of procedures for RNA-extraction from peripheral blood mononuclear cells — Europe PMC PMID:32084228

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/32084228
DOI: 10.1371/journal.pone.0229423
License (as archived): CC0-1.0
Archived: 2026-11-09T10:14:09.522Z
SHA-256 of the archived text: c2e7609ae50396477f9f708afde2d309718ff6f80577343e3b3e75bbde78ae2a
Stable URL of this page: https://metraipolis.com/documento/dc_2f9bebe5fe2e

How to cite

Comparison of procedures for RNA-extraction from peripheral blood mononuclear cells — Europe PMC PMID:32084228 — Europe PMC (en), https://europepmc.org/article/MED/32084228 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_2f9bebe5fe2e (Metraipolis archive dc_2f9bebe5fe2e, sha256 c2e7609ae50396477f9f708afde2d309718ff6f80577343e3b3e75bbde78ae2a)

BibTeX

@misc{dc2f9bebe5fe2e,
  title = {Comparison of procedures for RNA-extraction from peripheral blood mononuclear cells — Europe PMC PMID:32084228},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/32084228},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_2f9bebe5fe2e; sha256 c2e7609ae50396477f9f708afde2d309718ff6f80577343e3b3e75bbde78ae2a},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/32084228. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

RNA quality and quantity are important factors for ensuring the accuracy of gene expression analysis and other RNA-based downstream applications. Thus far, only a limited number of methodological studies have compared sample storage and RNA extraction procedures for human cells. We compared three commercially available RNA extraction kits, i.e., (NucliSENS) easyMAG, RNeasy (Mini Kit) and RiboPure (RNA Purification Kit-blood). In addition, additional conditions, such as storage medium and storage temperature of human peripheral blood mononuclear cells were evaluated, i.e., 4 °C for RNAlater or -80 °C for QIAzol and for the respective cognate lysis buffers; easyMAG, RNeasy or RiboPure. RNA was extracted from aliquots that had been stored for one day (Run 1) or 83 days (Run 2). After DNase treatment, quantity and quality of RNA were assessed by means of a NanoDrop spectrophotometer, 2100 Bioanalyzer and RT-qPCR for the ACTB reference gene. We observed that high-quality RNA can be obtained using RNeasy and RiboPure, regardless of the storage medium, whereas samples stored in RNAlater resulted in the least amount of RNA extracted. In addition, RiboPure combined with storage of samples in its cognate lysis buffer yielded twice as much RNA as all other procedures. These results were supported by RT-qPCR and by the reproducibility observed for two independent extraction runs.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PloS one, 2020 (prima pubblicazione 2020-02-21).
Identificativi: PMID 32084228 · PMCID PMC7034890 · DOI 10.1371/journal.pone.0229423.
Pagina: https://europepmc.org/article/MED/32084228 — https://doi.org/10.1371/journal.pone.0229423. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-11-09T10:08:47.539Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.