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Metabolomic and Transcriptomic Analysis of MCF-7 Cells Exposed to 23 Chemicals at Human-Relevant Levels: Estimation of Individual Chemical Contribution to… — Europe PMC PMID:33325755

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/33325755
DOI: 10.1289/ehp6641
License (as archived): CC0-1.0
Archived: 2026-11-06T15:58:36.291Z
SHA-256 of the archived text: adec47c298d7fbbf9461efd9a984be28b97e6515306755e7dd56c3d18b17e757
Stable URL of this page: https://metraipolis.com/documento/dc_2dbc9268ed2e

How to cite

Metabolomic and Transcriptomic Analysis of MCF-7 Cells Exposed to 23 Chemicals at Human-Relevant Levels: Estimation of Individual Chemical Contribution to… — Europe PMC PMID:33325755 — Europe PMC (en), https://europepmc.org/article/MED/33325755 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_2dbc9268ed2e (Metraipolis archive dc_2dbc9268ed2e, sha256 adec47c298d7fbbf9461efd9a984be28b97e6515306755e7dd56c3d18b17e757)

BibTeX

@misc{dc2dbc9268ed2e,
  title = {Metabolomic and Transcriptomic Analysis of MCF-7 Cells Exposed to 23 Chemicals at Human-Relevant Levels: Estimation of Individual Chemical Contribution to… — Europe PMC PMID:33325755},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/33325755},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_2dbc9268ed2e; sha256 adec47c298d7fbbf9461efd9a984be28b97e6515306755e7dd56c3d18b17e757},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/33325755. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

Background: Humans are constantly being exposed to various xenobiotics at relatively low concentrations. To date, limited evidence is available to ascertain whether a complex xenobiotic mixture at human-relevant levels causes any health effect. Moreover, there is no effective method to pinpoint the contribution of each chemical toward such an effect. Objectives: This study aims to understand the responses of cells to a mixture containing 23 xenobiotics at human-relevant levels and develop a feasible method to decipher the chemical(s) that contribute significantly to the observed effect. Methods: We characterized the metabolome and transcriptome of breast cancer cells (MCF-7) before and after exposure to the mixture at human-relevant levels; preexposure levels were derived from existing large-scale biomonitoring data. A high-throughput metabolomics-based "leave-one-out" method was proposed to understand the relative contribution of each component by comparing the metabolome with and without the particular chemical in the mixture. Results: The metabolomic analysis suggested that the mixture altered metabolites associated with cell proliferation and oxidative stress. For the transcriptomes, gene ontology terms and pathways including "cell cycle," "cell proliferation," and "cell division" were significantly altered after mixture exposure. The mixture altered genes associated with pathways such as "genotoxicity" and "nuclear factor erythroid 2-related factor 2 (Nrf2)." Through joint pathways analysis, metabolites and genes were observed to be well-aligned in pyrimidine and purine metabolisms. The leave-one-out results showed that many chemicals made their contributions to specific metabolic pathways. The overall metabolome pattern of the absence of 2,4-dihyroxybenzophenone (DHB) or bisphenol A (BPA) showed great resemblance to controls, suggesting their higher relative contribution to the observed effect. Discussion: The omics results showed that exposure to the mixture at human-relevant levels can induce significant in vitro cellular changes. Also, the leave one out method offers an effective approach for deconvoluting the effects of the mixture. https://doi.org/10.1289/EHP6641.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: Environmental health perspectives, 2020 (prima pubblicazione 2020-12-16).
Identificativi: PMID 33325755 · PMCID PMC7741182 · DOI 10.1289/ehp6641.
Descrittori MeSH principali: Toxicity Tests.
Pagina: https://europepmc.org/article/MED/33325755 — https://doi.org/10.1289/ehp6641. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-11-06T15:51:05.948Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.