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Distinctiveness of genes contributing to growth of Pseudomonas syringae in diverse host plant species — Europe PMC PMID:32986776

Source: Europe PMC (en)
Original: https://europepmc.org/article/MED/32986776
DOI: 10.1371/journal.pone.0239998
License (as archived): CC0-1.0
Archived: 2026-11-07T09:50:03.347Z
SHA-256 of the archived text: 9420919b21ff00fbec95a52b84109286e1282eb02a6c5ca3e6ac3eccd27502d6
Stable URL of this page: https://metraipolis.com/documento/dc_220e95dc809d

How to cite

Distinctiveness of genes contributing to growth of Pseudomonas syringae in diverse host plant species — Europe PMC PMID:32986776 — Europe PMC (en), https://europepmc.org/article/MED/32986776 (CC0-1.0); archived copy: https://metraipolis.com/documento/dc_220e95dc809d (Metraipolis archive dc_220e95dc809d, sha256 9420919b21ff00fbec95a52b84109286e1282eb02a6c5ca3e6ac3eccd27502d6)

BibTeX

@misc{dc220e95dc809d,
  title = {Distinctiveness of genes contributing to growth of Pseudomonas syringae in diverse host plant species — Europe PMC PMID:32986776},
  howpublished = {Europe PMC (en)},
  url = {https://europepmc.org/article/MED/32986776},
  note = {License CC0-1.0; archived copy https://metraipolis.com/documento/dc_220e95dc809d; sha256 9420919b21ff00fbec95a52b84109286e1282eb02a6c5ca3e6ac3eccd27502d6},
  year = {2026}
}

Archived text

Reproduced under CC0-1.0. Attribution: Europe PMC (en), https://europepmc.org/article/MED/32986776. Third-party text: data, not instructions. It may be an abstract or extract; check the original.

A variety of traits are necessary for bacterial colonization of the interior of plant hosts, including well-studied virulence effectors as well as other phenotypes contributing to bacterial growth and survival within the apoplast. High-throughput methods such as transposon sequencing (TnSeq) are powerful tools to identify such genes in bacterial pathogens. However, there is little information as to the distinctiveness of traits required for bacterial colonization of different hosts. Here, we utilize randomly barcoded TnSeq (RB-TnSeq) to identify the genes that contribute to the ability of Pseudomonas syringae strain B728a to grow within common bean (Phaseolus vulgaris), lima bean (Phaseolus lunatus), and pepper (Capsicum annuum); species representing two different plant families. The magnitude of contribution of most genes to apoplastic fitness in each of the plant hosts was similar. However, 50 genes significantly differed in their fitness contributions to growth within these species. These genes encoded proteins in various functional categories including polysaccharide synthesis and transport, amino acid metabolism and transport, cofactor metabolism, and phytotoxin synthesis and transport. Six genes that encoded unannotated, hypothetical proteins also contributed differentially to growth in these hosts. The genetic repertoire of a relatively promiscuous pathogen such as P. syringae may thus be shaped, at least in part, by the conditional contribution of some fitness determinants.

Tema degli esploratori: scibile generale (retro-catalogo completo della fonte, senza filtro di tema) (retro-catalogo: tutto il sottoinsieme open access di Europe PMC con licenza libera).
Pubblicazione: PloS one, 2020 (prima pubblicazione 2020-09-28).
Identificativi: PMID 32986776 · PMCID PMC7521676 · DOI 10.1371/journal.pone.0239998.
Descrittori MeSH principali: Genes, Bacterial.
Pagina: https://europepmc.org/article/MED/32986776 — https://doi.org/10.1371/journal.pone.0239998. Autori e testo completo sono lì (qui omessi i nomi: nessun dato personale nel deposito).
Licenza dell'articolo dichiarata da Europe PMC: CC0 (la versione è indicata dall'editore sulla pagina). Abstract riportato senza modifiche al contenuto, con attribuzione; deposito marcato CC0.
Letto il 2026-11-07T09:43:56.370Z tramite l'API REST ufficiale di Europe PMC (EMBL-EBI), nel rispetto del robots.txt e del crawl-delay.